期刊
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
卷 63, 期 3, 页码 519-525出版社
SPRINGER
DOI: 10.1007/s10971-012-2814-7
关键词
Lipase; Immobilization; N-(2-carboxylbenzoyl) chitosan; Reversible solubility
资金
- Open Project Program of State Key Laboratory of Food Science and Technology, Jiangnan University [SKLF-KF-201101]
- Key Laboratory of Bioresource Protection and Utilization of Anhui Province
- Key Laboratory of Biotic Environment and Ecological Safety of Anhui Province
- Program for Innovative Research Team in Anhui Normal University
N-(2-carboxylbenzoyl) chitosan (CBC), a reversibly soluble-insoluble polymer with pH change, was prepared by modifying chitosan backbone with phthalic anhydride and employed as carrier for lipase immobilization. The obtained CBC exhibited reversible solubility in aqueous solution; it was soluble at pH above 3.8 and precipitated at pH below 3.4. The porcine pancreatic lipase was covalently immobilized on CBC with glutaraldehyde as the crosslinking agent. Under the optimal immobilization condition, the retention activity of the immobilized lipase was found to be 69.8 %. The maximum activity of lipase immobilized on CBC was observed at 40 A degrees C, pH 8.0, while the free lipase presented maximum activity at 37 A degrees C, pH 7.5. The lipase immobilized on CBC exhibited improved thermal and storage stabilities and retained 58.7 % of its initial activity after 9 times of repeated use.
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