期刊
PLANT CELL
卷 27, 期 4, 页码 1082-1097出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.114.135160
关键词
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资金
- Deutsche Forschungsgemeinschaft [SCHA 1274/2-1, SCHA 1274/3-1, SFB 1101/TPA05]
- Deutscher Akademischer Austauschdienst
- Medical Research Council (MRC) [MC_U122680443]
- Swiss National Science Foundation [PZ00P2_136816]
- National Institutes of Health [R01CA107134]
- Vidi Grant of the Dutch Technology Foundation STW [11281]
- MRC [MC_U122680443, MC_UU_12018/4] Funding Source: UKRI
- Medical Research Council [MC_UU_12018/4, MC_U122680443] Funding Source: researchfish
- Swiss National Science Foundation (SNF) [PZ00P2_136816] Funding Source: Swiss National Science Foundation (SNF)
Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K- and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP(8) by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP(8) in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show high-affinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP(8) by COI1-JAZ is a critical component in jasmonate-regulated defenses.
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