期刊
PLANT CELL
卷 27, 期 7, 页码 1839-1856出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.15.00208
关键词
-
资金
- National Natural Science Foundation of China [31201220, 31123009, 31222041]
- National Program on Key Basic Research Project of China [2014CB138202]
- National Hi-Tech Research and Development Program of China [2012AA10A307]
- Deutsche Israeli Project [FE 552/12-1]
- EU-funded OPTICHINA project
Deciphering the influence of genetics on primary metabolism in plants will provide insights useful for genetic improvement and enhance our fundamental understanding of plant growth and development. Although maize (Zea mays) is a major crop for food and feed worldwide, the genetic architecture of its primary metabolism is largely unknown. Here, we use high-density linkage mapping to dissect large-scale metabolic traits measured in three different tissues (leaf at seedling stage, leaf at reproductive stage, and kernel at 15 d after pollination [DAP]) of a maize recombinant inbred line population. We identify 297 quantitative trait loci (QTLs) with moderate (86.2% of the mapped QTL, R-2 = 2.4 to 15%) to major effects (13.8% of the mapped QTL, R-2 > 15%) for 79 primary metabolites across three tissues. Pairwise epistatic interactions between these identified loci are detected for more than 25.9% metabolites explaining 6.6% of the phenotypic variance on average (ranging between 1.7 and 16.6%), which implies that epistasis may play an important role for some metabolites. Key candidate genes are highlighted and mapped to carbohydrate metabolism, the tricarboxylic acid cycle, and several important amino acid biosynthetic and catabolic pathways, with two of them being further validated using candidate gene association and expression profiling analysis. Our results reveal a metabolite-metabolite-agronomic trait network that, together with the genetic determinants of maize primary metabolism identified herein, promotes efficient utilization of metabolites in maize improvement.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据