期刊
JOURNAL OF SEPARATION SCIENCE
卷 35, 期 17, 页码 2313-2317出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200231
关键词
Brown algae; Fucoxanthin; High-speed countercurrent chromatography; Microwave-assisted extraction
资金
- Natural Science Foundation of China [20905080, 21127008]
- Guangdong Provincial Department of Science and Technology of China [2010B030600012]
- Fundamental Research Funds for the Central Universities [11lgpy80]
A rapid and efficient method for the separation and purification of fucoxanthin from edible brown algae by microwave-assisted extraction coupled with high-speed countercurrent chromatography was developed. The algae were first extracted using microwave-assisted extraction, then the dried extract was dissolved and directly introduced into the high-speed countercurrent chromatography system with a two-phase solvent system consisting of hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v/v/v). The isolation was done in less than 75 min, and a total of 0.83 mg, 1.09 mg, and 0.20 mg fucoxanthin were obtained from 25.0 g fresh Laminaria japonica Aresch, 1.5 g dry Undaria pinnatifida (Harv) Sur, and 15.0 g dry Sargassum fusiforme (Harv) Setch, respectively. The purity of fucoxanthin determined by HPLC was over 90% and its structure was further identified by LC-ESI-MS and 1H-NMR.
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