期刊
JOURNAL OF SEPARATION SCIENCE
卷 35, 期 9, 页码 1102-1109出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201101094
关键词
Corydaline metabolism; Human hepatocytes; Human liver microsomes; LC-MSn
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2011-0018631]
- Office of Strategic R&D Planning (OSP)
- Ministry of Knowledge Economy, Republic of Korea [10039303]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10039303] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Corydaline is a pharmacologically active isoquinoline alkaloid isolated from Corydalis tubers. It exhibits the antiacetylcholinesterase, antiallergic, antinociceptive, and gastric emptying activities. The purposes of this study were to establish in vitro metabolic pathways of corydaline in human liver microsomes and hepatocytes by identification of their metabolites using liquid chromatography-ion trap mass spectrometry. Human liver microsomal incubation of corydaline in the presence of an NADPH-generating system resulted in the formation of nine metabolites, namely, four O-desmethylcorydaline [M1 (yuanhunine), M2 (9-O-desmethylcorydaline), M3 (isocorybulbine), and M4 (corybulbine)], three di-O-desmethylcorydaline [M5 (9,10-di-O-desmethylcorydaline), M6 (2,10-di-O-desmethylcorydaline), and M7 (3,10-di-O-desmethylcorydaline)], M8 (hydroxyyuanhunine), and M9 (hydroxycorydaline). Incubation of corydaline in human hepatocytes produced four metabolites including M1, M5, M6, and M9. O-Demethylation and hydroxylation were the major metabolic pathways for the metabolism of corydaline in human liver microsomes and hepatocytes.
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