期刊
JOURNAL OF SEPARATION SCIENCE
卷 35, 期 14, 页码 1723-1730出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201101092
关键词
High-temperature HPLC; Solvent compatibility; Solvent effects; Solvent strength; Two-dimensional liquid chromatography
资金
- German Federal Ministry of Economics and Technology within the agenda for the promotion of industrial cooperative research and development (IGF)
- AiF, Arbeitsgemeinschaft industrieller Forschungsvereinigungen, Cologne, Germany [15928 N]
Solvent compatibility is a limiting factor for the success of two-dimensional liquid chromatography (2-D LC). In the second dimension, solvent effects can result in overpressures as well as in peak broadening or even distortion. A peak shape study was performed on a one-dimensional high-performance liquid chromatography (HPLC) system to simulate the impact of peak distorting solvent effects on a reversed-phase second dimension separation operated at high temperatures. This study includes changes in injection volume, solute concentration, column inner diameter, eluent composition and oven temperature. Special attention was given to the influence of high temperatures on the solvent effects. High-temperature HPLC (HT-HPLC) is known to enhance second dimension separations in terms of speed, selectivity and solvent compatibility. The ability to minimise the viscosity contrast between the mobile phases of both dimensions makes HT-HPLC a promising tool to avoid viscosity mismatch effects like (pre-)viscous fingering. In case of our study, viscosity mismatch effects could not be observed. However, our results clearly show that the enhancement in solvent compatibility provided by the application of high temperatures does not include the elimination of solvent strength effects. The additional peak broadening and distortion caused by this effect is a potential error source for data processing in 2-D LC.
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