期刊
JOURNAL OF SEPARATION SCIENCE
卷 34, 期 18, 页码 2448-2454出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201100267
关键词
Aminoglycosides; CE-capacitively coupled contactless conductivity detection Kanamycin; Pharmaceutical analysis; Quality control
A method was developed to determine simultaneously kanamycin, its related substances and sulphate in kanamycin sulphate using capacitively coupled contactless conductivity detection. Kanamycin is an aminoglycoside antibiotic that lacks a strong UV-absorbing chromophore. Due to its physicochemical properties, CE in combination with capacitively coupled contactless conductivity detection was chosen. The separation method uses a BGE composed of 40 mM 2-(N-morpholino)ethanesulphonic acid monohydrate and 40 mM L-histidine, pH 6.35. A 0.6 mM N-cetyltrimethyl ammonium bromide (CTAB) solution was added as electroosmotic flow modifier in a concentration below the critical micellar concentration (CMC). Ammonium acetate 50 mg/L was used as internal standard. In total, 30 kV was applied in reverse polarity on a fused-silica capillary (65/41 cm; 75 mu m id). The optimized separation was obtained in less than 6 min with good linearity (R-2 = 0.9999) for kanamycin. It shows a good precision expressed as RSD on the relative peak areas equal to 0.3 and 1.1% for intra-day and inter-day precision, respectively. The LOD and LOQ are 0.7 and 2.3 mg/L, respectively. Similarly, for sulphate, a good linearity (R-2 = 0.9996) and precision (RSD 0.4 and 0.6% for intra-day and inter-day, respectively) were obtained.
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