Functionalized polymeric stationary phases for ion chromatography

Synthesis and properties of the multilayered stationary phases, which contain quaternary amine functional groups for the analysis of anions by ion chromatography, are described. The bonded phases were characterized by elemental analysis, solid-state C-13 NMR spectroscopy and chromatographic methods. The surface of 1,4-di(2-hydroxy-3-methacryloyloxypropoxy) phenol (solid support) was coated with polymeric layers formed by condensation polymerization of primary amine with diepoxide. Each layer of the anion exchange stationary phase consists of copolymer of methylamine (MA) and 1,4-butanedioldiglycidyl ether (BDDE). A series of stationary phases with different numbers of polymerized layers were tested. The separation of an inorganic anions sample (F-, Cl-, NO2-, Br-, NO3-, additionally HPO42- and SO42-) was performed. In the measurement, a hydroxide, carbonate, bicarbonate and their mixture were used as mobile phases.