期刊
JOURNAL OF SEPARATION SCIENCE
卷 34, 期 5, 页码 500-507出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000723
关键词
Antiretroviral drugs; Gradient elution; Ionic liquids; Mobile phase additives; Monolith column
资金
- University Grants Commission (UGC)
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
The use of 3-methylimidazolium cation-based ionic liquids (ILs) was evaluated as mobile phase additives for separation of antiretroviral drugs on a monolithic column by RP-HPLC. Separation of eight commonly used antiretroviral drugs was achieved on a Chromolith Flash, RP-18e column (25 x 4.6 mm, porous material) using water (pH 4.0 adjusted with acetic acid)/methanol v/v as a mobile phase containing ILs in a gradient elution mode. The effects of concentrations of ILs on retention, resolution and peak shape were studied and a regression equation correlating the interactions between stationary phase and the ILs was established. The retention of all the drugs was decreased notably by using 1-butyl-3-methylimidazolium tetrafluoroborate, while 1-ethyl-3-methylimidazolium methylsulfate reduced gradient drift drastically when compared to triethylamine.
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