期刊
JOURNAL OF SEPARATION SCIENCE
卷 34, 期 5, 页码 594-600出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000686
关键词
Capillary electrophoresis; Colchicine; Ionic liquid; Online coupling; Single-drop microextraction
资金
- National Natural Science Foundation of China [20905073, 20905072, 20975105]
- Technology R&D Program of Gansu Province of China [0804GKCA034]
A novel approach based on ionic liquid-single-drop microextraction (IL-SDME) online coupling with capillary electrophoresis (CE) was used to determine a toxic alkaloid - colchicine. The IL-SDME procedure was optimized by extraction solvent, drop volume controlling, sample volume and pH, extraction time, and ionic strength. Under optimum conditions, enrichment factor was as much as 41-fold with a relative standard deviation of 2.8% (n = 3). Linear range of response was observed from 1 to 100 mu g/mL, with detection limit of 0.25 mu g/mL and correlation coefficient (R-2) of 0.9994. The extraction of colchicine from spiked Lanzhou lily sample was performed and obtaining good result with an average recovery rate of 102.4 and 98.8% at 5 and 50 mu g/mL, respectively. Comparing with the previous methods, IL-SDME-CE is really a convenient, economical, and environmentally benign way for determining colchicine.
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