期刊
JOURNAL OF SEPARATION SCIENCE
卷 34, 期 18, 页码 2371-2375出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201100445
关键词
17 beta-Estradiol; Estriol; Estrone; Environmental waters; HPLC
资金
- Wenzhou University
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
A simple, sensitive and accurate reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of three naturally occurring estrogenic steroids including estrone (E1), 17 beta-estradiol (E2) and estriol (E3) in environmental water samples was developed. Analytes were extracted with ethyl acetate solvents and preconcentrated prior to HPLC analysis. Separations were accomplished in <20 min using a reversed-phase C-18 column (4.6 x 250mm id, 5 mu m) with a gradient elution of mobile phase containing 3.0 mM ammonium acetate/acetonitrile mixtures (flow rate, 1.0 mL/min). UV light absorption responses at 205 nm were linear over a wide concentration range from 100 000 mu g/L to the detection limits of 0.96 mu g/L E1, 0.64 mu g/L E2 and 0.78 mu g/L E3. Quantitation was carried out by the peak area method. The relative standard deviation for the analysis of three estrogens was <3.0%. This method was applied for the simultaneous determination of estrogens in environmental water samples collected in Zhejiang, China. The higher concentrations of both E2 and E3 were found in Tang River and West Lake waters, and E1 was detected in lake water only. All three estrogens were below the detection limits in rain waters.
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