Determination of ofloxacin and lomefloxacin in chicken muscle using molecularly imprinted solid-phase extraction coupled with liquid chromatography

A new molecularly imprinted solid-phase extraction (MISPE) procedure combined with liquid chromatography was developed for the simultaneous selective extraction and determination of ofloxacin (OFL) and lomefloxacin (LOM) in chicken muscle samples. The water-compatible molecularly imprinted microspheres (MIMs) were synthesized by aqueous suspension polymerization using 2-hydroxy-3-naphthoic acid and 1-methylpiperazine as mimic templates. The MIMs applied as selective sorbents in SPE method showed high selectivity and affinity to OFL and LOM in complex biological matrices. Good linearity was obtained in a range of 0.025-2.0 mu g/g, and the average recoveries of OFL and LOM at three spiked levels ranged from 94.4 to 96.9%, respectively, with the relative standard deviation <= 4.7%. The developed MISPE-HPLC method was successfully applied to the isolation of OFL and LOM in chicken muscles, which demonstrated the potential ability of the novel MIMs for selective extraction of fluoroquinolones in biological samples.