期刊
JOURNAL OF SEPARATION SCIENCE
卷 33, 期 15, 页码 2361-2368出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000224
关键词
Ceria-zirconia composite; Dephosphorylation; Enrichment; MALDI-TOF-MS; Phosphopeptide
资金
- National Science Fund for Distinguished Young Scholars [20625516]
- Science Fund for Creative Research Groups [20921062]
- National 973 project of China [2007CB914200]
Ceria-zirconia composites at different molar ratios were synthesized. Several methods were used to characterize these composites, including X-ray photoelectron spectroscopy, surface area and surface acid-base property detection. A one-step method for isolation and identification of phosphopeptides from peptide mixture was created using these ceria-zirconia composites. Using tryptic digest of standard phosphorylated protein, we have shown that these enrichment and dephosphorylation activities are effective. The adsorption capacity and catalytic property of ceria-zirconia composites at different molar ratios and calcinated temperatures were studied. In combination with MALDI-TOF-based peptide mass finger printing technique, we have established a method to utilize the enrichment/dephosphorylation dual properties of these ceria-zirconia composites for the analysis of phosphoprotein in nonfat milk successfully.
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