期刊
JOURNAL OF SEPARATION SCIENCE
卷 33, 期 17-18, 页码 2804-2810出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000211
关键词
Coated capillary; N-Vinylimidazole; Reversed electroosmotic flow; Surface-initiated atom transfer radical polymerization
资金
- National Natural Science Foundation of China [20775084, 20875096, 20805052]
A CE method for poly(N-vinylimidazole) (PVI)-grafted capillaries by the surface-initiated atom transfer radical polymerization has been developed. The coating was prepared with N-vinylimidazole as the monomer, 2-bromo-2-methyl-N-[3-(triethoxysilyl) propyl] propanamide (BTPAm) as the initiator and CuCl/CuCl2/2,2'-bipyridine as the catalyst and ligand. The direction and magnitude of EOF in the PVI-grafted capillary were investigated in a pH range of 3.0-9.0. The results indicated that the EOF could be modulated by varying the pH value of the buffer and an anodic EOF was obtained at pH values below 6.5. A significant improvement in reproducibility and reduction of EOF appeared on the PVI-grafted capillary when compared with the uncoated capillary. Furthermore, the polymer coated capillaries were applied to the separations of the inorganic anions, organic acids and basic proteins and baseline separations were achieved with short analysis time and high reproducibility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据