期刊
JOURNAL OF SEPARATION SCIENCE
卷 33, 期 23-24, 页码 3751-3758出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201000430
关键词
Analysis; Apple pomace; Microwave-assisted extraction; Polyphenols; Response surface methodology
资金
- Tackling Key Science & Technology of Shaanxi Province [2008K08-04]
- Specific Innovation Project for Important Science & Technology of Shaanxi Province [2008ZDKG-04]
A simple and efficient microwave-assisted extraction of polyphenols from industrial apple pomace was developed and optimized by the maximization of the yield using response surface methodology. A Box-Behnken design was used to monitor the effect of microwave power, extraction time, ethanol concentration and ratio of solvent to raw material (g/mL) on the polyphenols yield. The results showed that the optimal conditions were as follows: microwave power 650.4 W, extraction time 53.7 s, ethanol concentration 62.1% and ratio of solvent to raw material 22.9:1. Validation tests indicated that the actual yield of polyphenols was 62.68 +/- 0.35 mg gallic acid equivalents per 100 g dry apple pomace with RSD = 0.86% (n = 55) under the optimal conditions, which was in good agreement with the predicted yield and higher than those of reflux and ultrasonic-assisted extraction methods. HPLC analysis indicated that the major polyphenols of apple pomace consisted of chlorogenic acid, caffeic acid, syrigin, procyanidin B2, (-)-epicatechin, cinnamic acid, coumaric acid, phlorizin and quercetin, of which procyanidin B2 had the highest content of 219.4 mg/kg.
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