期刊
JOURNAL OF SEPARATION SCIENCE
卷 33, 期 4-5, 页码 600-609出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900695
关键词
Deans Switch; Deoxynivalenol (DON); Heart-cutting GC-MS; QuEChERS; Trichothecenes; Zearelenone (ZON)
资金
- FCT [PTDC/AGR-ALI/101583/2008]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-ALI/101583/2008] Funding Source: FCT
A new analytical method for the rapid and simultaneous determination of five mycotoxins (zearelenone, deoxynivalenol, Fusarenon X, 15-acetyldeoxynivalenol and nivalenol) in breakfast cereals and flours by heart-cutting GC-MS has been developed and validated. Extraction was performed with MeCN, applying a modified QuEChERS (QUick, Easy, CHeap, Effective, Rugged and Safe) procedure, and the extracts were analyzed after a silylation of the analytes under study. Careful optimization of the parameters of Deans Switch device and GC-MS was achieved in order to attain a fast separation in SIM mode, allowing a total run time of only 8 min. Acceptable recoveries for all mycotoxins at two different spiking levels (20 and 100 mu g/kg) were achieved with good repeatability (from 9 to 21%). LOD ranged from 2 to 15 mu g/kg and LOQ ranged from 5 to 50 mu g/kg. which were lower than the maximum limit legal established by the European Union (EU). The method developed was applied to commercial breakfast cereals and flours; among the mycotoxins studied, deoxynivalenol and zearalenone were the most predominant.
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