期刊
JOURNAL OF SEPARATION SCIENCE
卷 32, 期 20, 页码 3441-3448出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900298
关键词
Anthocyanins; DAD; ESI-TOF-MS; Hibiscus sabdariffa; HPLC; IT-MS; Polyphenols
资金
- Spanish Ministry of Education and Science [AGL2008-05108-003-03]
- Andalusian Regional Government Council of Innovation and Science [P07-AGR-02619]
The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.
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