期刊
JOURNAL OF SEPARATION SCIENCE
卷 32, 期 5-6, 页码 835-853出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800640
关键词
alpha(1)-Acid glycoprotein; Drug-protein binding; High-performance affinity chromatography; Human serum albumin (HSA); Lipoproteins
资金
- NIDDK NIH HHS [R01 DK069629, R01 DK069629-03] Funding Source: Medline
- NIGMS NIH HHS [R01 GM044931, R01 GM044931-16] Funding Source: Medline
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK069629] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM044931] Funding Source: NIH RePORTER
The binding of drugs with proteins in blood, serum, or plasma is an important process in determining the activity, distribution, rate of excretion, and toxicity of drugs in the body. High-performance affinity chromatography (HPAC) has received a great deal of interest as a means for studying these interactions. This review examines the various techniques that have been used in HPAC to examine drug-protein binding and discusses the types of information that can be obtained through this approach. A comparison of these techniques with traditional methods for binding studies (e.g., equilibrium dialysis and ultrafiltration) will also be presented. The use of HPAC with specific serum proteins and binding agents will then be discussed, including HSA and alpha(1)-acid glycoprotein (AGP). Several examples from the literature are provided to illustrate the applications of such research. Recent developments in this field are also described, such as the use of improved immobilization techniques, new data analysis methods, techniques for working directly with complex biological samples, and work with immobilized lipoproteins. The relative advantages and limitations of the methods that are described will be considered and the possible use of these techniques in the high-throughput screening or characterization of drug-protein binding will be discussed.
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