期刊
JOURNAL OF SEPARATION SCIENCE
卷 32, 期 15-16, 页码 2565-2573出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900255
关键词
Capillary liquid chromatography; Cation-exchange chromatography; Peptides and proteins; Polymer monolith
资金
- Pfizer Analytical RD
A strong cation-exchange (SCX) monolithic stationary phase was prepared in 75 mu m id capillaries by direct in situ polymerization of sulfopropyl methacrylate and polyethylene glycol diacrylate in a ternary porogen system consisting of methanol, cyclohexanol, and water. The resulting monolith exhibited good dynamic binding capacity, fast kinetic adsorption of proteins, and high permeability. The monolith had a dynamic binding capacity of similar to 52 mg/mL of column volume for lysozyme and cytochrome C. The monolith was evaluated for SCX capillary LC of synthetic peptides, natural peptides, and protein standards. Fast separation of proteins was achieved in less than 4 min. The average peak capacity for peptides was 28 using a relatively steep gradient when hydrophobic interactions were suppressed with 40% acetonitrile.
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