期刊
PLANT AND CELL PHYSIOLOGY
卷 56, 期 5, 页码 951-964出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcv022
关键词
ABA accumulation; Lowland and upland rice; pH; Root system; Sclerenchyma layer; Water stress
资金
- Shenzhen Overseas Talents Innovation and Entrepreneurship Funding Scheme [The Peacock Scheme]
- National Natural Science Foundation of China [NSFC31301251, NSFC91317307, NSFC31101099]
- Hong Kong Research Grants Council [AoE/M-05/12]
- Shenzhen Vegetable Molecular Biotechnological Engineering Lab [Development & Reform Commission of Shenzhen Municipal Government]
Lowland rice (Nipponbare) and upland rice (Gaoshan 1) that are comparable under normal and moderate drought conditions showed dramatic differences in severe drought conditions, both naturally occurring long-term drought and simulated rapid water deficits. We focused on their root response and found that enhanced tolerance of upland rice to severe drought conditions was mainly due to the lower level of ABA in its roots than in those of the lowland rice. We first excluded the effect of ABA biosynthesis and catabolism on root-accumulated ABA levels in both types of rice by monitoring the expression of four OsNCED genes and two OsABA8ox genes. Next, we excluded the impact of the aerial parts on roots by suppressing leaf-biosynthesized ABA with fluridone and NDGA (nordihydroguaiaretic acid), and measuring the ABA level in detached roots. Instead, we proved that upland rice had the ability to export considerably more root-sourced ABA than lowland rice under severe drought, which improved ABA-dependent drought adaptation. The investigation of apoplastic pH in root cells and root anatomy showed that ABA leakage in the root system of upland rice was related to high apoplastic pH and the absence of Casparian bands in the sclerenchyma layer. Finally, taking some genes as examples, we predicted that different ABA levels in rice roots stimulated distinct ABA perception and signaling cascades, which influenced its response to water stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据