期刊
JOURNAL OF SEPARATION SCIENCE
卷 32, 期 10, 页码 1523-1530出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800695
关键词
Aptamers; Antibodies; Epitopes; SELEX
资金
- FAPESP (Fundacao de Amparo Pesquisa do Estado de Sao Paulo)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazil
- Deutsche Forschungsgemeinschaft [WR 124/2]
RNA and DNA aptamers developed by systematic evolution of ligands by exponential enrichment (SELFA) have turned into important tools in diagnostics, research, and therapeutics. Unlike antibodies, high-affinity and specific aptamers identified through an in vitro selection process can be chemically modified to gain nuclease resistances in biological fluids and to extend their bioavailability in animals. Aptamers can be raised against virtually any target including those which are toxic or do not elicit any immune response in animals. They can be developed in automated processes against various protein targets and then easily modified by attaching fluorescence reporters, nanoparticles or biotin moieties, rival antibodies in high-throughput proteomics and cell separations. In this review, we will discuss the high competence of aptamers in recognizing biomarkers and molecular signatures of cell surfaces, and how these unique features can be exploited for the identification and isolation of cancer, stem cells and even detection of parasite-infected cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据