期刊
JOURNAL OF SEPARATION SCIENCE
卷 31, 期 21, 页码 3727-3731出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800350
关键词
Alkaloids; Micellar electrokinetic chromatography; SPE
资金
- Food and Drug Administration (FDA)
- Naval Academy Research Council
- Office of Naval Research
Implementation of an uncomplicated SPE process for the rapid extraction and pre-concentration of the alkaloids, colchicine, strychnine, aconitine, and nicotine, from water, apple juice, and nonfat milk samples is presented. When Coupled to analysis via micellar EKC (MEKC), the total analysis time per sample was less than 15 min for the water and juice samples and less than 20 min for the milk, The SPE process allowed for anywhere from a three to a fourteen-fold improvement in the LOD for each alkaloid when compared to detecting the alkaloids in a nontreated water sample matrix. Following SPE, the LODs for colchicine, strychnine, and nicotine were sufficient to meet levels from 150 to 5000 times more dilute than the LD50 for a 50 kg individual drinking 12 oz of a contaminated beverage. Aconitine, on the other hand, was detected at approximately the LD50 level. The percent recoveries for the SPE ranged from 37% to as high as 99%. Nicotine attained the highest recovery efficiencies, followed by colchicine, and finally, aconitine and strychnine, which were nearly identical. The greatest recovery efficiencies were achieved from apple juice and water, whereas nonfat milk yielded the lowest.
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