期刊
JOURNAL OF SEPARATION SCIENCE
卷 31, 期 15, 页码 2868-2874出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800085
关键词
amoxicillin; cephalexin; molecularly imprinted polymer; solid-phase extraction; urine samples
资金
- Ministry of Education and Science [CTM 2005-01774, CTM 2004-06265-03]
- Departament d'Innovacio, Universtat i Empresa de la Generalitat de Catalunya and the Fons Social Europeu [2007FI_B 01357]
In this paper we describe, for the first time, a molecularly imprinted polymer (MIP) for the antibiotic amoxicillin (AMX), synthesised by a noncovalent molecular imprinting approach and used to extract AMX selectively from urine samples. The MIP was applied as a molecularly selective sorbent in molecularly imprinted SPE (MISPE) in an off-line mode, where it showed useful cross-selectivity for a structurally related antibiotic, cephalexin (M). By using a MISPE protocol, the MIP was able to selectively extract both AMX and CFX from 5 mL of water spiked with 10 mg/L with recoveries of 75 and 78% for AMX and CFX, respectively. When applied to real samples (urine) at clinically relevant concentrations, recoveries from 2 mL of human urine spiked with 20 mg/L decreased slightly to 65 and 63% for AMX and CFX, respectively. To demonstrate further the selectivity of the MIP obtained, a comparison with commercially available SPE cartridges was performed. Improvements in the retention of both AMX and CFX on the MIP were obtained relative to the commercially available cartridges, and the MISPE extracts were considerably cleaner, due to molecularly selective analyte binding by the MIP.
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