期刊
JOURNAL OF SEPARATION SCIENCE
卷 31, 期 19, 页码 3466-3472出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800287
关键词
Comprehensive two-dimensional liquid chromatography; Phenolic compounds; Ultra performance liquid chromatography; Wine
资金
- Academy of Finland [110429]
- Academy of Finland (AKA) [110429, 110429] Funding Source: Academy of Finland (AKA)
In this study, 1-D and 2-D liquid chromatographic systems, namely, conventional HPLC, UPLC, HPLC x HPLC and HPLC x UPLC systems were developed and evaluated for the separation of phenolic acids in wine and juices. In the LC x LC studies, the first dimension separation was based on RPLC and the second dimension was performed with ion-pair chromatography. Three different columns, namely two short columns packed with either 2.5 or 1.7 mu m particles and a monolithic column, were tested for the fast second dimension separation. The best results were obtained when the monolithic column was applied for the second dimension separation. The peak capacities for comprehensive 2-D systems varied from 330 to 616.
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