期刊
JOURNAL OF SEPARATION SCIENCE
卷 31, 期 15, 页码 2804-2812出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200800247
关键词
3-(4-chlorobenzoyl)-quinoline-2-carboxaldehyde; biogenic amines; derivatization; food sample; high performance liquid chromatography
资金
- National Natural Science Foundation of China, Beijing, China [20575047, 30770550 and 20775058]
A simple HPLC approach has been successfully established for the sensitive determination of six biogenic amines (BAs) in food samples. The method involves derivatization with 3-(4-chlorobenzoyl)-quinoline-2-carboxaldehyde newly synthesized as a new fluorogenic reagent followed by LC isocratic elution mode. The optimization of both derivatization and separation conditions is carefully studied. Related analyses of the eluted compounds, in the presence of MeOH/THF/H2O (78:2.5:19.5 v/v/v) as a mobile phase containing 8 mM, pH 6.0 phosphate buffer solution, have been carried Out Oil a C,, column. The LOD (S/N = 3) of 2.5 nM, RSD value from 1.0 to 5.1% in peak areas, and good response linearity (R-2 > 0.9936) are provided with fluorescence detection at lambda(ex)/lambda(em) = 480/545 nm. Obviously, recovery ranging from 95 to 107% in this method demonstrates its accuracy for determination of histamine, tyramine, 2-phenylethylamine, putrescine, cadaverine, and spermidine in the storage fish sample. Thus, the present method could be developed to monitor BAs in fish, Cucumber, and spinach samples.
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