期刊
PHYTOTHERAPY RESEARCH
卷 29, 期 7, 页码 1088-1096出版社
WILEY
DOI: 10.1002/ptr.5354
关键词
Saussurea lappa; alantolactone; chemokines; STAT1; keratinocytes
资金
- Korea Institute of Oriental Medicine [K15250]
- National Research Council of Science & Technology (NST), Republic of Korea [K15250] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Skin inflammation is the most common condition seen in dermatology practice and can be caused by various allergic reactions and certain toxins or chemicals. In the present study, we investigated the antiinflammatory effects of Saussurea lappa, a medicinal herb, and its marker compounds alantolactone, caryophyllene, costic acid, costunolide, and dehydrocostuslactone in the HaCaT human keratinocyte cell line. HaCaT cells were stimulated with tumor necrosis factor-alpha (TNF-) and interferon-gamma (IFN-), and treated with S.lappa or each of five marker compounds. Chemokine production and expression were analyzed by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction, respectively. Phosphorylation of signal transducer and activator of transcription (STAT) 1 was determined by immunoblotting. Stimulation with TNF- and IFN- significantly increased the production of the following chemokines: thymus-regulated and activation-regulated chemokine (TARC): regulated on activation, normal T-cell expressed and secreted (RANTES): macrophage-derived chemokine (MDC): and interleukin-8 (IL-8). By contrast, S.lappa and the five marker compounds significantly reduced the production of these chemokines by TNF- and IFN--treated cells. S.lappa and alantolactone suppressed the TNF- and IFN--stimulated increase in the phosphorylation of STAT1. Our results demonstrate that alantolactone from S.lappa suppresses TNF- and IFN--induced production of RANTES and IL-8 by blocking STAT1 phosphorylation in HaCaT cells. Copyright (c) 2015 John Wiley & Sons, Ltd.
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