期刊
JOURNAL OF REPRODUCTION AND DEVELOPMENT
卷 57, 期 3, 页码 421-427出版社
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
DOI: 10.1262/jrd.11-007H
关键词
Bcl-2-associated X protein (Bax); BH3-interacting domain death agonist (Bid); Follicular atresia; Mitochondrion-dependent type II apoptotic cell; Pig ovary
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [13GS0008]
- Japan Society for the Promotion of Science [18658105, 21658092, B18380164, B22380148, S16108003]
- Grants-in-Aid for Scientific Research [18658105, 13GS0008, 21658092] Funding Source: KAKEN
More than 99% of follicles undergo atresia during follicular development and growth. Follicular atresia is predominantly regulated by granulosa cell apoptosis. However, the intracellular signaling pathway of apoptosis in granulosa cells has not been revealed. In the present study, we examined changes in the expression of BH3-interacting domain death agonist (Bid) and Bcl-2-associated X protein (Bax), which are considered to promote the cell death ligand/receptor-mediated process in mitochondrion-dependent type IT apoptosis, in porcine granulosa cells during atresia. Levels of mRNA and protein of Bid and Bax were determined by the reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting techniques, respectively. Levels of Bid and Bax mRNA and protein were markedly increased in granulosa cells of early atretic follicles compared with those of healthy follicles. In situ hybridization and immunohistochemical staining revealed that mRNA and protein of Bid and Bax were present in the granulosa cells, though only traces were found in healthy follicles; however, strong staining was noted in atretic follicles. These results indicate that Bid and Bax appear to be signal transduction factors in granulosa cells during follicular atresia and appear to play proapoptotic roles and confirm that the porcine granulosa cell is a mitochondrion-dependent type II apoptotic cell.
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