期刊
PHYTOCHEMISTRY
卷 113, 期 -, 页码 140-148出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2014.10.013
关键词
Pea; Pisum sativum; Fabaceae; Fusarium solani f. sp phaseoli; Dirigent protein; Pinoresinol; Pisatin; Pinoresinol monoglucoside; MALDI mass spectrometry imaging; Gene expression; Phytoalexin; Non-host disease response
资金
- U.S. National Science Foundation [MCB-1052557]
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy [DE-FG-0397ER20259]
- G. Thomas and Anita Hargrove Center for Plant Genomic Research
- National Science Foundation [DBI-1229749]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1229749] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1052557] Funding Source: National Science Foundation
Continually exposed to potential pathogens, vascular plants have evolved intricate defense mechanisms to recognize encroaching threats and defend themselves. They do so by inducing a set of defense responses that can help defeat and/or limit effects of invading pathogens, of which the non-host disease resistance response is the most common. In this regard, pea (Pisum sativum) pod tissue, when exposed to Fusarium solani f. sp. phaseoli spores, undergoes an inducible transcriptional activation of pathogenesis-related genes, and also produces (+)-pisatin, its major phytoalexin. One of the inducible pathogenesis-related genes is Disease Resistance Response-206 (DRR206), whose role in vivo was unknown. DRR206 is, however, related to the dirigent protein (DP) family. In this study, its biochemical function was investigated in planta, with the metabolite associated with its gene induction being pinoresinol monoglucoside. Interestingly, both pinoresinol monoglucoside and (+)-pisatin were co-localized in pea pod endocarp epidermal cells, as demonstrated using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. In addition, endocarp epidermal cells are also the site for both chalcone synthase and DRR206 gene expression. Taken together, these data indicate that both (+)-pisatin and pinoresinol monoglucoside function in the overall phytoalexin responses. (C) 2014 Elsevier Ltd. All rights reserved.
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