期刊
PHYTOCHEMISTRY
卷 115, 期 -, 页码 121-129出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2015.02.019
关键词
Arabidopsis thaliana; Brassicaceae; Pollen; Sphingolipid; Lipidomics; Glucosylceramide; Glycosylinositolphosphoceramide
资金
- U.S. National Science Foundation [MCB-1158500]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1158500] Funding Source: National Science Foundation
- EPSCoR
- Office Of The Director [1004094] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005207] Funding Source: researchfish
- BBSRC [BBS/E/C/00005207] Funding Source: UKRI
Although sphingolipids are essential for male gametophytic development in Arabidopsis thaliana, sphingolipid composition and biosynthetic gene expression have not been previously examined in pollen. In this report, electrospray ionization (ESI)-MS/MS was applied to characterization of sphingolipid compositional profiles in pollen isolated from wild type Arabidopsis Col-0 and a long-chain base (LCB) Delta 4 desaturase mutant. Pollen fractions were highly enriched in glucosylceramides (GlcCer) relative to levels previously reported in leaves. Accompanying the loss of the Delta 4 unsaturated LCB sphingadiene (d18:2) in the Delta 4 desaturase mutant was a 50% reduction in GlcCer concentrations. In addition, pollen glycosylinositolphosphoceramides (GIPCs) were found to have a complex array of N-acetyl-glycosylated GIPCs, including species with up to three pentose units that were absent from leaf GIPCs. Underlying the distinct sphingolipid composition of pollen, genes for key biosynthetic enzymes for GlcCer and d18:2 synthesis and metabolism were more highly expressed in pollen than in leaves or seedlings, including genes for GlcCer synthase (GCS), sphingoid base C-4 hydroxylase 2 (SBH2), LCB Delta 8 desaturases (SLD1 and SLD2), and LOH2 ceramide synthase (LOH2). Overall, these findings indicate strikingly divergent sphingolipid metabolism between pollen and leaves in Arabidopsis, the significance of which remains to be determined. (C) 2015 Elsevier Ltd. All rights reserved.
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