期刊
PHYTOCHEMICAL ANALYSIS
卷 27, 期 1, 页码 64-72出版社
WILEY-BLACKWELL
DOI: 10.1002/pca.2600
关键词
Ricinus communis; low molecular weight metabolites; analysis strategy; NMR; HPLC; LC-MS
资金
- Organisation for the Prohibition of Chemical Weapons (OPCW) [L/ICA/ICB/185625/13]
Introduction - Powder-like extract of Ricinus communis seeds contain a toxic protein, ricin, which has a history of military, criminal and terroristic use. As the detection of ricin in this terrorist powder is difficult and time-consuming, related low mass metabolites have been suggested to be useful for screening as biomarkers of ricin. Objective - To apply a comprehensive NMR-based analysis strategy for annotation, isolation and structure elucidation of low-molecular weight plant metabolites of Ricinus communis seeds. Methodology - The seed extract was prepared with a well-known acetone extraction approach. The common metabolites were annotated from seed extract dissolved in acidic solution using H-1 NMR spectroscopy with spectrum library comparison and standard addition, whereas unconfirmed metabolites were identified using multi-step off-line HPLC-DAD-NMR approach. Results - In addition to the common plant metabolites, two previously unreported compounds, 1,3-digalactoinositol and ricinyl-alanine, were identified with support of MS analyses. Conclusion - The applied comprehensive NMR-based analysis strategy provided identification of the prominent low molecular weight metabolites with high confidence. Copyright (C) 2015 John Wiley & Sons, Ltd.
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