期刊
JOURNAL OF RAMAN SPECTROSCOPY
卷 42, 期 11, 页码 1913-1915出版社
WILEY-BLACKWELL
DOI: 10.1002/jrs.2952
关键词
Raman microspectroscopy; label-free imaging; biofilms; leucine
类别
资金
- National Chiao Tung University
- Ministry of Education (Taiwan)
- National Science Council of Taiwan [NSC97-2113-M-009-002-MY2]
In structured communities of bacteria known as biofilms, a variety of biomolecules have been shown to play a unique role as signals and/or regulators in biofilm formation. Here, we report that high levels of the amino acid leucine ( leucine pool) were detected, for the first time, within microcolonies in a 30-h-old Escherichia coli biofilm by Raman imaging. Localization of leucine revealed by multifrequency Raman images indicates leucine accumulation during the early stage of the E. coli biofilm formation, which may have resulted from physiological environment-specific metabolic adaptation. We demonstrate that our label-free Raman imaging method provides a useful platform for directly identifying still unknown natural products produced in biofilms as well as for visualizing heterogeneous distributions of biofilm constituents in situ. Copyright (C) 2011 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据