期刊
JOURNAL OF RAMAN SPECTROSCOPY
卷 43, 期 8, 页码 971-976出版社
WILEY
DOI: 10.1002/jrs.3124
关键词
FT-SERS; adenine; hypoxanthine; xanthine; Raman spectrometry; nucleotides; nanoparticle
类别
资金
- Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.05/2.1.00/03.0058]
Surface-enhanced Raman scattering spectroscopy represents one of the unique techniques for studying nanoscale objects, and its distinctive properties can be used in the process of further analysis. The careful evaluation of the particular influence of selected key-role experimental parameters (e.g. pH value of measured sample mixture, size and distribution of used nanoparticles) and the influence of reduction agent used in the process of formation of desired nanoparticle objects presents an important task in the further study of surface-enhanced Raman scattering effect. A broad study of these experimental parameters was performed in this paper. The main aim of the presented work was to a demonstrate an application potential of selected experimental conditions in the determination of three purine bases: adenine, xanthine, and hypoxanthine. The resulting limits of detection are at femtomolar concentration levels for all three studied compounds. Copyright (c) 2011 John Wiley & Sons, Ltd.
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