期刊
JOURNAL OF RAMAN SPECTROSCOPY
卷 43, 期 2, 页码 187-195出版社
WILEY-BLACKWELL
DOI: 10.1002/jrs.3029
关键词
SERS; RNA; nucleosides; aggregating agent; molecular spectroscopy
类别
资金
- Engineering and Physical Sciences Research Council [GR/S75727/01]
- University of Manchester
- Royal Society of Chemistry Analytical Trust [GR/T05554/01]
- Engineering and Physical Sciences Research Council [GR/S75727/01] Funding Source: researchfish
We present surface-enhanced Raman scattering (SERS) spectra of the RNA nucleosides adenosine, cytidine, guanosine and uracil and the methylated derivatives 5-methylcytidine and 7-methylguanosine, a class of important nucleic acid components that has not previously been well characterised using SERS spectroscopy. Our work shows that the selection of aggregating agent plays a crucial role for SERS analysis of these nucleosides with K2SO4 generating immediate enhancement while NaCl only gave immediate enhancement for the pyrimidine nucleosides. The SERS spectra contain a number of marker bands that are highly sensitive to structural differences between these nucleosides, in particular methylation, and at lower concentration ranges than are possible for conventional Raman scattering. Finally, spectral analyses and assignments of the vibrational modes responsible for these marker bands are also presented, and the effect of the aggregating agent on these modes is discussed in terms of interactions between each nucleoside and the metal surface. Copyright (C) 2011 John Wiley & Sons, Ltd.
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