Development of chromatographic separation of some biological materials using poly(acrylamide-acrylic acid-dimethylaminoethyl methacrylate) resin

The aim of the present study is to develop of chromatographic separation of some biological materials using poly(acrylamide-acrylic acid-dimethylaminoethyl methacrylate) resin P(AAm-AA-DMAEMA). The copolymer was prepared by a template copolymerization of DMAEMA and AA in aqueous solution on polyacrylamide (PAAm) as a template polymer and N,N-methylenebisacrylamide (NMBA) as a crosslinker using gamma rays as initiator. Some biological materials such as alpha-fetoprotein (AFP), bovine serum albumin (BSA) and tri-iodothyronine (T(3)) were labeled using (125)I and the purifications of all tracers were carried out using prepared resin compared with Sephadex. Factors affecting were studied to reach the most efficiency purification including pH buffer, variable elution volumes, flow rate and temperature. The radiochemical purity percent was determined using paper electrophoresis and immunoreactivity of tracer was checked. The efficiency of purification that obtained using the prepared resin is nearly equivalent to that of Sephadex.