期刊
JOURNAL OF PROTEOMICS
卷 78, 期 -, 页码 254-272出版社
ELSEVIER
DOI: 10.1016/j.jprot.2012.09.021
关键词
Phaseolus vulgaris; Drought stress; Proteomics; Protein network
资金
- Slovenian Research Agency [J4-4126, P4-0072]
- Slovenian Fellowship Programme
- Norwegian Financial Mechanism for mobility of researchers
The majority of common bean plants are cultivated under drought conditions. Maintaining crop yields under drought stress is thus one of the biggest challenges facing bean production. In order to improve our understanding of the complex mechanisms involved in the response of common bean (Phaseolus vulgaris) to drought stress, a proteomic approach was used to identify drought-responsive proteins in leaves of two cultivars differing in their response to drought, Tiber and more sensitive Starozagorski cern. 2D-DIGE was used to compare differences in protein abundance between control and stressed plants. Fifty-eight proteins whose abundance changed significantly were identified by LC-MS/MS in Tiber and 64 in Starozagorski cern. The majority of identified proteins were classified into functional categories that include energy metabolism, photosynthesis, ATP interconversion, protein synthesis and proteolysis, stress and defence related proteins. Details of the function of the identified proteins and their abundance profiles in Tiber and Starozagorski are discussed. Interactions between identified proteins were demonstrated by bioinformatics analysis, enabling a more complete insight into biological pathways and molecular functions affected by drought stress. The results form the basis for a further understanding of the biochemical mechanisms of drought response in common bean. (C) 2012 Elsevier B.V. All rights reserved.
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