期刊
JOURNAL OF PROTEOMICS
卷 75, 期 16, 页码 5036-5051出版社
ELSEVIER
DOI: 10.1016/j.jprot.2012.03.017
关键词
Secondary ion mass spectrometry; Matrix assisted laser desorption/ionization; Subcellular profiling; Elemental imaging; Mass cytometry; Tissue imaging
资金
- National Institute on Drug Abuse (NIDA) [P30 DA018310]
- Department of Energy [DE-SC0006642]
- U.S. Department of Energy (DOE) [DE-SC0006642] Funding Source: U.S. Department of Energy (DOE)
Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling. This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research. (C) 2012 Elsevier B.V. All rights reserved.
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