期刊
JOURNAL OF PROTEOMICS
卷 75, 期 12, 页码 3478-3494出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2012.04.030
关键词
Protein-RNA complexes; Mass spectrometry; Crosslinking; Quantitative mass spectrometry; Native mass spectrometry
Protein-RNA complexes play many important roles in diverse cellular functions. They are involved in a wide variety of different processes in growth and differentiation at the various stages of the cell cycle. As their function and catalytic activity are directly coupled to the structural arrangement of their components-proteins and ribonucleic acids-the investigation of protein-RNA interactions is of great functional and structural importance. Here we discuss the most prominent examples of protein-RNA complexes and describe some frequently used purification strategies. We present various techniques and applications of mass spectrometry to study protein-RNA complexes. We discuss the analysis of intact complexes as well as proteomics-based and crosslinking-based approaches in which proteins are cleaved into smaller peptides. This article is part of a Special Section entitled: Understanding genome regulation and genetic diversity by mass spectrometry. (c) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据