期刊
JOURNAL OF PROTEOMICS
卷 75, 期 16, 页码 5014-5026出版社
ELSEVIER
DOI: 10.1016/j.jprot.2012.03.015
关键词
Imaging mass spectrometry; MALDI; Neurons; Neuroendocrine tissues; Sampling; Neuropeptides
资金
- National Science Foundation [CHE-0957784]
- National Institutes of Health [1R01DK071801, R56DK071801]
- H. I. Romnes Faculty Fellowship
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [957784] Funding Source: National Science Foundation
Imaging mass spectrometry (IMS) has evolved to be a promising technology due to its ability to detect a broad mass range of molecular species and create density maps for selected compounds. It is currently one of the most useful techniques to determine the spatial distribution of neuropeptides in cells and tissues. Although IMS is conceptually simple, sample preparation steps, mass analyzers, and software suites are just a few of the factors that contribute to the successful design of a neuropeptide IMS experiment. This review provides a brief overview of IMS sampling protocols, instrumentation, data analysis tools, technological advancements and applications to neuropeptide localization in neurons and endocrine tissues. Future perspectives in this field are also provided, concluding that neuropeptide IMS would greatly facilitate studies of neuronal network and biomarker discovery. This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research. (C) 2012 Elsevier B.V. All rights reserved.
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