期刊
JOURNAL OF PROTEOMICS
卷 75, 期 2, 页码 628-641出版社
ELSEVIER
DOI: 10.1016/j.jprot.2011.09.002
关键词
Proteomics; Blood-brain barrier; Cytoskeletal remodelling; Triton X-100 soluble proteins
资金
- Ministere de la Recherche et de l'Enseignement Superieur
- Oseo-Anvar
- European Regional Development Fund
- Fonds d'Industrialisation des Bassins Miniers (FIBM
- Ministere de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
- Universite d'Artois
When in the vicinity of astrocytes, brain capillary endothelial cells (BCECs) develop the characteristic structural and functional features of the blood-brain barrier (BBB). The latter has low cellular permeability and restricts various compounds from entering the brain. We recently reported that the cytoskeleton-related proteins actin, gelsolin and filamin-A undergo the largest quantitative changes in bovine BCECs after re-induction of BBB functions by coculture with glial cells. In the present study, we used an in-depth, proteomic approach to quantitatively compare differences in Triton-X-100-solubilized proteins from bovine BCECs with limited or re-induced BBB functions (i.e. cultured in the absence or presence of glial cells, respectively). The 81 protein spots of differing abundance were linked to 55 distinct genes. According to the Protein ANalysis THrough Evolutionary Relationships classification system and an Ingenuity Pathway Analysis, these quantitative changes mainly affected proteins involved in (i) cell structure and motility and (ii) protein metabolism and modification. The fold-changes affecting HSPB1, moesin and ANXA5 protein levels were confirmed by western blot analysis but were not accompanied by changes in the corresponding mRNA expression levels. Our results reveal that the bovine BCECs' phenotype adaptation to variations in their environment involves the reorganization of the actin cytoskeleton. (C) 2011 Elsevier B.V. All rights reserved.
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