期刊
JOURNAL OF PROTEOMICS
卷 74, 期 8, 页码 1411-1420出版社
ELSEVIER
DOI: 10.1016/j.jprot.2011.03.017
关键词
Medicago truncatula; Extracellular proteins; Wounding; Reactive oxygen species; Apoplast proteome; MALDI-TOF/TOF
资金
- Instituto de Biologia Experimental e Tecnologica (Plant-IBET initiative)
- Fundacao para a Ciencia e a Tecnologia [SFRH/BD/6487/2001]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/6487/2001] Funding Source: FCT
Wounded Medicago truncatula leaves produce a burst of O-2(-) (phase I) between 1 and 15 mm, then of O-2(-) and H2O2 (phase II) between 1 and 3 h. Our previous results suggest reactive oxygen species (ROS) may provide signals to mobilise early (6 h), apoplastic, wound-responsive proteins (WRPs). 2DE and MALDI-TOF/TOF were used to analyse how the suppression of ROS production at different time points by diphenyleneiodonium (DPI), affects the expression of WRPs. Rapid (<= 3 min) DPI inhibition of phase I O-2(-) production suppressed the differential regulation of 7 out of 19 WRPs, which were consequently classified as ROS-dependent WRPs. DPI inhibition of only phase II ROS production failed to suppress the wound regulation of 18 out of 19 WRPs, but led to the altered expression of 1 ROS-dependent WRP and 2 non-WRPs (Group B). The data indicates Group B proteins are alternatively targeted via the modulation of phase II ROS production. This reinforces an important role for phase I O-2(-) signalling in the early wound response, but indicates that this response is partly regulated by phase II of the oxidative burst. This data provides an informed basis for further proteomic studies aimed at identifying early activated O-2(-) signalling components in wounded Medicago. (C) 2011 Elsevier B.V. All rights reserved.
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