期刊
JOURNAL OF PROTEOMICS
卷 74, 期 6, 页码 894-901出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2011.03.006
关键词
Brain proteome; Variation; Mouse strain; Two-dimensional electrophoresis
资金
- Hungarian National Sciences Foundation [OTKA NK 73672]
- Memo load [FP-7 201159]
- Lipididiet [FP-7 211696]
- TAMOP [4.2.2]
Neuroproteomics is aimed to study the molecular organisation of the nervous system at the protein level. Two-dimensional electrophoresis is the most frequently used technique in quantitative proteomics. The aim of this study was to assess the experimental and biological variations on this proteomic platform using mouse brain tissue. Mice are the most generally used lab animals for modelling human disease or investigating the effect of a drug-candidate or a treatment. Experimental design plays a crucial role in quantitative proteomics, hence understanding and minimizing the variables is essential. Our results indicate that the technical variance dominantly contributes to the total variance in mouse brain and the genetic background has a negligible effect on the total variation. The results also characterise the anticipated variation using mouse brain for proteomic study hence they should be useful for future experimental design in other proteomics laboratories. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据