期刊
JOURNAL OF PROTEOMICS
卷 74, 期 7, 页码 1045-1067出版社
ELSEVIER
DOI: 10.1016/j.jprot.2011.03.009
关键词
Rice; Salt stress; Apoplast protein; 2-D DIGE; LC-MS/MS
资金
- National Science Foundation of China [30870200, 31071245]
- Key project of Chinese National Transgenic Program [2009ZX08009-017B]
- Natural Science Foundation of the Hebei Province in China [C2008000171]
- Hebei Province Foundation for Returned Scholars [20100327]
- NIH National Center for Research Resources [P41RR001614, RR019934]
Plants have evolved sophisticated systems to cope with adverse environmental conditions such as cold, drought, and salinity. Although a number of stress response networks have been proposed, the role of plant apoplast in plant stress response has been ignored. To investigate the role of apoplastic proteins in the salt stress response, 10-day old rice plants were treated with 200 mM NaCl for 1, 6 or 12 h, and the soluble apoplast proteins of rice shoot stems were extracted for differential analysis, compared with untreated controls, by 2-D DIGE saturation labeling techniques. One hundred twenty-two significantly changed spots were identified by LC-MS/MS, and 117 spots representing 69 proteins have been identified. Of these proteins, 37 are apoplastic proteins according to the bioinformatic analysis. These proteins are mainly involved in the processes of carbohydrate metabolism, oxido-reduction, and protein processing and degradation. According to their functional categories and cluster analysis, a stress response model of apoplastic proteins has been proposed. These data indicate that the apoplast is important in plant stress signal reception and response. (C) 2011 Elsevier B.V. All rights reserved.
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