期刊
JOURNAL OF PROTEOMICS
卷 73, 期 5, 页码 868-878出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2010.01.005
关键词
Membrane protein analysis; Blue Native electrophoresis; Protein-lipid interaction; Metabolic stable isotope labeling
资金
- Netherlands Proteomics Centre
The analysis of proteins in biological membranes forms a major challenge in proteomics Despite continuous improvements and the development of more sensitive analytical methods, the analysis of membrane proteins has always been hampered by their hydrophobic properties and relatively low abundance In this review, we describe recent successful strategies that have led to in-depth analyses of the membrane proteome To facilitate membrane proteome analysis, it is essential that biochemical enrichment procedures are combined with special analytical workflows that are all optimized to cope with hydrophobic polypeptides These include techniques for protein solubilization, and also well-matched developments in protein separation and protein digestion procedures Finally, we discuss approaches to target membrane-protein complexes and lipid-protein interactions, as such approaches offer unique insights into function and architecture of cellular membranes (C) 2010 Elsevier B V All rights reserved
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