期刊
JOURNAL OF PROTEOMICS
卷 73, 期 5, 页码 899-916出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2009.12.001
关键词
Manganese; Microarray; Neisseria gonorrhoeae; Oxidative stress; Pili; Proteomics
资金
- National Health and Medical Research Council of Australia [284214]
- National Science Council [NSC 93-3112B-001-010-Y]
- Academia Sinica
- Institute of Biological Chemistry, Academia Sinica, Taiwan
- NHMRC
- USPHS/NIH [AI38399]
- Cooperative Human Tissue Network (Columbus, Ohio, USA)
- Columbus Children's Research Institute
Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn) We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag ([ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e g, pilin, a key adhesin), oxidative stress defence (e g, superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance (C) 2009 Published by Elsevier B V
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据