期刊
JOURNAL OF PROTEOMICS
卷 72, 期 1, 页码 110-120出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2008.10.008
关键词
Multidimensional protein identification technology; Normalized spectral abundance factor; Quantitative proteomics; Nucleus; Transcription factors; Protein complexes
资金
- NIGMS [F32 GM075541]
- Stowers Institute for Medical Research
To gain insight into the nuclear proteome of Saccharomyces cerevisiae, nuclei were isolated and fractionated via sucrose gradient sedimentation. The resulting fractions were analyzed using multidimensional protein identification technology and the detected proteins were quantified using normalized spectral counts. A large number of low abundance proteins, many of which are involved in transcriptional regulation, were recovered. Sucrose gradient elution profiles of known protein complex components demonstrated that this approach may provide insight into the question of what percentage of the total population of a protein is in one complex, versus another protein complex, or exists as a free protein. (C) 2008 Elsevier B.V. All rights reserved.
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