期刊
JOURNAL OF PROTEOME RESEARCH
卷 13, 期 12, 页码 5777-5783出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr5006907
关键词
milk enzymes; enzyme activity; digestive enzymes; infant digestion; proteolytic enzymes; human milk; indigenous enzymes
资金
- Enterprise Ireland grant [CC20080001]
- Science Foundation Ireland [08/IN.1/B1864]
- University of California Discovery Program [05GEB01NHB]
- National Science Foundation
- USDA National Institute for Food and Agriculture
- National Institutes of Health [R01 HD059127, UL1 TR000002]
Little is known about the digestive process in infants. In particular, the chronological activity of enzymes across the course of digestion in the infant remains largely unknown. To create a temporal picture of how milk proteins are digested, enzyme activity was compared between intact human milk samples from three mothers and the gastric samples from each of their 4-12 day postpartum infants, 2 h after breast milk ingestion. The activities of 7 distinct enzymes are predicted in the infant stomach based on their observed cleavage pattern in peptidomics data. We found that the same patterns of cleavage were evident in both intact human milk and gastric milk samples, demonstrating that the enzyme activities that begin in milk persist in the infant stomach. However, the extent of enzyme activity is found to vary greatly between the intact milk and gastric samples. Overall, we observe that milk-specific proteins are cleaved at higher levels in the stomach compared to human milk. Notably, the enzymes we predict here only explain 78% of the cleavages uniquely observed in the gastric samples, highlighting that further investigation of the specific enzyme activities associated with digestion in infants is warranted.
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