期刊
JOURNAL OF PROTEOME RESEARCH
卷 13, 期 8, 页码 3693-3705出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr5002065
关键词
basement membrane; collagen IV; laminin; nidogens; perlecan; collagens; proteomics; mass spectrometry
Basement membranes (BMs) are extracellular matrix sheets comprising the laminins, type-IV collagens, nidogens, and the heparan sulfate proteoglycans, perlecan, collagen XVIII, and agrin. In intact BMs, BM proteins are physiologically insoluble and partially resistant to proteolytic digestion, making BMs a challenge to study. Here three types of BMs from adult human eyes, the inner limiting membrane (ILM), the retinal vascular BMs, and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS. Peptide and protein identifications were done using MaxQuant. 1129 proteins were identified with a 1% false discovery rate. Data showed that BMs are composed of multiple laminins, collagen IVs, nidogens, and proteoglycans. The dominant laminin family member in all BMs was laminin alpha 5 beta 2 gamma 1. The dominant collagen IV trimer in lens capsule (LC) and blood vessel (BV) BMs had a chain composition of alpha 1(IV)(2), alpha 2 (IV), whereas the dominant collagen IV in the ILM had the alpha 3(IV), alpha 4(IV), alpha 5(IV) chain composition. The data also showed that the ratio of laminin and collagen varied among different BM types: the ratio of collagen IV to the other BM proteins is highest in LC, followed by BV and lowest for the ILM. The data have been deposited to the ProteomeXchange with identifier PXD001025.
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