期刊
JOURNAL OF PROTEOME RESEARCH
卷 13, 期 5, 页码 2282-2296出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr401204y
关键词
Metarhizium anisopliae; shotgun proteomics; biocontrol; host infection; Dysdercus peruvianus
资金
- National Center for Research Resources [5P41RR011823-17]
- National Institute of General Medical Sciences [8 P41 GM103533-17]
- National Heart, Lung, and Blood Institute [HHSN268201000035C]
- National Institute on Aging [R01AG027463-04]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae.
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