期刊
JOURNAL OF PROTEOME RESEARCH
卷 12, 期 11, 页码 5281-5292出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr4006316
关键词
DIGE; cyanobacteria; Roundup; proteomics; bivalve; Unionid
资金
- European University of Brittany (UEB), Rennes, France
Cyanobacterial toxins and pesticides regularly impact fresh-waters. Microcystin-LR is one of the most toxic and common cyanobacterial toxins whereas glyphosate is the active ingredient of a widely use herbicide. As filter feeders, freshwater mussels are particularly exposed. Like many native bivalve species, Unio pictorum suffers from a continuous decline in Europe. In order to get a deeper insight of its response to contaminants, U. pictorum was exposed to either 10 mu g L-1 of microcystin-LR or 10 mu g L-1 of glyphosate or a mixture of both. Proteins of the digestive glands were extracted and analyzed by DIGE. Gel analysis revealed 103 spots with statistical variations, and the response seems to be less toward glyphosate than to microcystin-LR. Specific spots have variations only when exposed to the mixture, showing that there is an interaction of both contaminants in the responses triggered. The proteins of 30 spots have been identified. They belong mostly to the cytoskeleton family, but proteins of the oxidative pathway, detoxification, and energetic metabolism were affected either by glyphosate or microcystin-LR or by the mixture. These results demonstrate the importance to study contaminants at low concentrations representative of those found in the field and that multicontaminations can lead to different response pathways.
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