期刊
JOURNAL OF PROTEOME RESEARCH
卷 12, 期 12, 页码 5934-5942出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr400279u
关键词
TransCAT; MetCAT; fusion construct; triple-quadrupole mass spectrometry; insoluble fusion expression protein; NUSa-soluble fusion expression protein; SDS-PAGE
资金
- Khaled Hassan Al-Ghamdi Est.
- Manchester Pharmacy School
QconCAT is a tool for quantitative proteomics, consisting of an artificial protein, expressed from an artificial gene, made up of a concatenated string of proteotypic peptides selected from the proteins under study. Isotopically labeled QconCAT (usually containing C-13(6)-arginine and C-13(6)-lysine) provides a standard for each proteotypic peptide included in its sequence. In practice, some QconCAT proteins fail to express at sufficient levels for the purpose of quantitative analysis. Two complementary methods are presented to express recalcitrant QconCAT proteins intended to quantify human hepatic enzymes and transporters.
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